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NOTE:
DO NOT ALLOW THIS TESTER TO BE IN
DIRECT SUNLIGHT DURING TEST INCUBATION.
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Obtain a representative
sample of wastewater from an aerated basin or tank (mixed-liquor
suspended solids, MLSS).
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Dilute sample 1:10,
1:100 or greater with sterile, non-chlorinated water to reduce
color interference from solids as well as possible chemical
interferences*. Use standard dilution bottles (100 ml capacity
or greater) or other sterile containers with lids or caps.
Dilution is also necessary to count viable active organisms
in samples exceeding counts of 10 8 colony-forming
units (CFU's) per milliliter (ml).
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Mix diluted sample
gently in the appropriate container for 20 to 30 seconds to
evenly disperse floc particles.
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Rinse snapping cup
three times with sample or sterile water. Fill Kool Kount
Assayer snapping cup with prepared sample and snap vial. Allow
vial to fill completely before removing.
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Incubate in a 35oC/95oF
environment. This may be done in a shirt breast pocket or
incubator. A special Carry/Incubation Tube (Catalog
#IM93357BK) is also
available to allow safe, convenient incubation.
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To stop a test, simply
refrigerate or put sample in an ice chest. To restart, simply
incubate and subtract 0.5 hour from the total cumulative time
to obtain final results.Read chart results times dilution
factor for final results.
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Obtain a sample from
the system to be tested. If sample is clear and is suspected
to contain 10 8 CFU/ml or less, simply fill snapping
cup and snap the Kool Kount Assayer ampoule until completely
filled. If sample is suspected to contain higher counts, or
contains chemical interferences or significant color, simply
dilute per instructions in Steps 2 and 3 above. Control ampoules
are also available for slight color adjustments.
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Proceed to Step 4
above and complete final steps.
*Chemical interferences may be due
to a strong presence of reducing compounds and high levels
of ammonia.
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