IME Inc.

IME.Test™ - KOOL KOUNT Assayer 3.5
Total Viable Aerobic Microbe Tester
Industrial & Cooling Tower Waters

RESULTS in HOURS not Days after Sampling

Standard TTC/TSB Based BioChemistry
No Storage Limitations • Long Shelf Life
RETAINS Test Sample

ASTM APPROVED
#D6530

IME.TEST™ - Kool Kount Assayer - 3.5 (KKA-3.5) Test contains a different concentration of TTC when compared to the regular Kool Kount Assayer TTC/TSB biochemistry. However, KKA-3.5 has all the attributes of the normal KKA product to include the same end of test color change to pink. The altered biochemistry of the KKA-3.5 is designed to optimize microbial concentration assessment in cooling tower waters without the use of chemical neutralizing agents. When compared to standard plate count for cooling tower water, KKA-3.5 has consistently generated better recovery rates than the chemical neutralization recovery process. The focus point of the test calibration is for the range of 104-105 cfu/ml* concentration levels. The KKA-3.5 calibration curve was developed and confirmed in conjunction with a nationwide supplier of water treatment chemicals. Species dominant waters (ie: Pseudomonus species) require a
one - time simple calibration adjustment of 2 hours. UV illumination and or test growth patterns can often field identify Pseudomonus species. Each KKA-3.5 comes complete with a sample/ampoule, snapping cup, dechlorinating solution, sample ID labels, and results/instruction chart. KKA-3.5 test ampoules can be purchased in boxes of 30 or as single test (Micro Inspector). Test incubation is normally at 95° F but room temperature incubations are possible. Incubation can be performed manually by purchasing a reusable carry incubation tube or using a standard laboratory heat block or oven. Automatic incubation and end of test detection can be accomplished by purchasing our low cost Incubator/AutoAnalyzer (model 2012). Special test calibrations/formulations and private labeling are available upon request.

*10,000 - 1000,000 cfu/ml

U.S. Patent #:
5159799
5550032

Box of 30 Test Ampoules
Product Code #: IM30077
EASY to USE

MANUAL
VISUAL
METHOD

or

AUTOMATIC
INSTRUMENT


Start
(yellow)

END
(lt.pink)

Run 2 Tests
Simultaneously

KOOL KOUNT Assayer 3.5 KIT DATA
TEST KIT SHELF LIFE - 4 years
# TESTS per TEST KIT: 30
TEST KIT WEIGHT - 14 ounces
TEST KIT SIZE - 3.25” x 2.75” x 5.25”
TEST KIT CONTAINER - Fiber box
TEST KIT TRANSPORTATION HAZARD Classification - None (DOT Ground)
TEST KIT CONTENTS: A kit contains 30 test ampoules, 1 snapping cup, dechlorinating solution, sample ID labels, Waste Water instructions and a results/instruction chart. Negative control ampoules, sample swabs and buffered sample solution must be ordered separately. When the 30 tests have been used, no replacement components are required, just order a new kit.

TEST NEUTRALIZES: Mercurial, iodine, chlorine, formaldehyde, Glutaraldehyde, Quaternary ammonium compounds, phenols, hexachlorophene, formalin and lecithin ethanol.

MANUAL TEST KIT OPERATION STEPS:
  1. Using the water to be tested, rinse the Sample/Snapping Cup 3 times and then fill with sample water.

  2. Place an IME.Test™ Ampoule tip down into one of the holes in the bottom of the filled Sample/Snapping Cup and pull back on the top to break the ampoule tip (see Step 1 on the other side of this sheet). The IME.Test™ Ampoule will automatically fill.

  3. Gently rock the filled IME.Test™ Ampoule back and forth for 30 seconds (longer if the sample is cold water) to mix the test reagent. After mixing, place IME.Test™ Ampoule in a 95 (F) degree environment (use KKA safety incubation tube in shirt pocket). Periodically, compare the ampoule color to the color card or negative control ampoule to determine end of test and test results. Or, select a control limit and look once at the IME.Test™ Ampoule.

RESULTS TABLES

TIME (Hrs.)
CONCENTRATION
COUNT
GENERAL
cfu/ml
0.5
10(8)
100,000,000
2.5
10(7)
10,000,000
4.0
10(6)
1,000,000
5.5
10(5)
100,000
7.0
10(4)
10,000
9.0
10(3)
1,000
10.0
10(2)
100
12.5
10(1)
10



Incubation Tube

AUTOMATIC TEST KIT OPERATION STEPS:
  1. Break the IME.Test™ Ampoule that you would like to run the test on.
  2. Dry the well mixed (no undissolved powder) Ampoule.
  3. INSERT the Ampoule into one of the test cells and close the cell cover.
  4. Press the "Cell" button until the display screen is showing the cell number you are using.
  5. Press the "Mode" button until the desired incubation mode is reached. (Total Bacteria, Total Coliform, E. Coli, Sulfate Bacteria, Fecal Coliform)
  6. Press "Start" button.
  7. Press "Enter" to begin the test.
  8. The test is finished when the display screen shows hours and count. (Example: 10.0 2e5, this indicates a test time of 10 hours and a count of 2 x 105)

Incubator/Autoanalyzer

USING as SAMPLER: If the IME.Test™ Ampoule is kept at a cool temperature, Biological growth will be delayed until the sample is brought to a proper incubation temperature. This feature can be used to take a sample at site and forward a proper sample to a lab. Or, if an abnormal field result is observed, the IME.Test™ Ampoule contents can be used for additional analysis (Biological or Chemical).

PRIVATE LABEL/CUSTOM TEST KITS: Private label and/or Customized TEST KITS are available upon request.


Automatic Incubator/Analyzer / Automatic Incubator/Analyzer / Coliform / Dental
E. Coli / Iron Bacteria / Micro Inspector / Potable Water / Sulfur Reducing Bacteria / Sulfur Reducing Bacteria-P
Total Microbe Family / Kool Kount / Kool Kount-P / Kool Kount 3.5/ Kool Kount .05 / Total Sulfur Bacteria / Total Sulfur Bacteria-P

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