IME Inc.

IME.Test™ - KOOL KOUNT Assayer
Total Viable Aerobic Microbe Tester
Environmental & General Analysis

RESULTS in HOURS not Days after Sampling

Standard TTC/TSB Based BioChemistry
Long Shelf Life • No Storage Limitations
RETAINS Test Sample

ASTM APPROVED
#D6530

Each IME.TEST™ - Kool Kount Assayer (KKA) Test is a pre-dosed, hermetically sealed, vacuum ampoule. The vacuum packaging of KKA preserves the reagent/media for years and requires no special storage conditions such as refrigeration. When the KKA test is started, a 7.5 ml aqueous sample is automatically drawn into the test ampoule. The test is concluded when the KKA ampoule turns orange or red. The elapsed time from test start to test end determines the level of microbial contamination (see reverse side). Test results come as fast as 1 hour for concentrations of 10(8) or 12 hours for 10(1) microbial concentrations. Or, KKA can be used as a presence/absence test at 24 hours. The TTC indicator reacts to all aerobic microbial activity in the sample to include facultative species. Fungi are also detected and indicated by floating red particles after 24 hours. Time/Concentration calibrations are based upon mixed microbial populations normally found in industrial and natural waters. Species dominant waters require a one-time, simple to perform calibration adjustment. Each KKA comes complete with a sample/ampoule, snapping cup, dechlorinating solution, sample ID labels, Waste Water instructions and a results/ instruction chart. KKA test ampoules can be purchased in boxes of 30 or as single test (Micro Inspector). Test incubation is normally at 95° F but room temperature incubations are possible. Test incubation can be performed manually by purchasing a reusable carry incubation tube or using a standard laboratory heat block or oven. Automatic incubation and end of test detection can accomplished by purchasing our low cost Incubator/AutoAnalyzer (model 2012). Special factory prepared test calibrations/formulations and/or private labeling are available upon request.

U.S. Patent #:
5159799
5550032

Box of 30 Test Ampoules
Product Code #: IM95077
EASY to USE

MANUAL
VISUAL
METHOD

or

AUTOMATIC
INSTRUMENT


Start
(yellow)

END
(orange)

Run 2 Tests
Simultaneously

 

KOOL KOUNT Assayer KIT DATA
TEST KIT SHELF LIFE - 4 years
# TESTS per TEST KIT: 30
TEST KIT WEIGHT - 14 ounces
TEST KIT SIZE - 3.25” x 2.75” x 5.25”
TEST KIT CONTAINER - Fiber box
TEST KIT TRANSPORTATION HAZARD Classification - None (DOT Ground)
TEST KIT CONTENTS: A kit contains 30 test ampoules, 1 snapping cup, dechlorinating solution, sample ID labels, Waste Water instructions and a results/instruction chart. Negative control ampoules, sample swabs and buffered sample solution must be ordered separately. When the 30 tests have been used, no replacement components are required, just order a new kit.
MANUAL TEST KIT OPERATION STEPS:
  1. Using the water to be tested, rinse the Sample/Snapping Cup 3 times and then fill with sample water.

  1. Place an IME.Test™ Ampoule tip down into one of the holes in the bottom of the filled Sample/Snapping Cup and pull back on the top to break the ampoule tip (see Step 1 on the other side of this sheet). The IME.Test™ Ampoule will automatically fill.

  2. Gently rock the filled IME.Test™ Ampoule back and forth for 30 seconds (longer if the sample is cold water) to mix the test reagent. After mixing, place IME.Test™ Ampoule in a 95 (F) degree environment (use KKA safety incubation tube in shirt pocket). Periodically, compare the ampoule color to the color card or negative control ampoule to determine end of test and test results. Or, select a control limit and look once at the IME.Test™ Ampoule.

RESULTS TABLES

TIME (Hrs.)
CONCENTRATION
COUNT
GENERAL
cfu/ml
0.5
10(8)
100,000,000
2.5
10(7)
10,000,000
4.0
10(6)
1,000,000
5.5
10(5)
100,000
7.0
10(4)
10,000
9.0
10(3)
1,000
10.0
10(2)
100
12.5
10(1)
10



Incubation Tube

AUTOMATIC TEST KIT OPERATION STEPS:
  1. Break the IME.Test™ Ampoule that you would like to run the test on.
  2. Dry the well mixed (no undissolved powder) Ampoule.
  3. INSERT the Ampoule into one of the test cells and close the cell cover.
  4. Press the "Cell" button until the display screen is showing the cell number you are using.
  5. Press the "Mode" button until the desired incubation mode is reached. (Total Bacteria, Total Coliform, E. Coli, Sulfate Bacteria, Fecal Coliform)
  6. Press "Start" button.
  7. Press "Enter" to begin the test.
  8. The test is finished when the display screen shows hours and count. (Example: 10.0 2e5, this indicates a test time of 10 hours and a count of 2 x 105)

Incubator/Autoanalyzer

USING as SAMPLER: If the IME.Test™ Ampoule is kept at a cool temperature, Biological growth will be delayed until the sample is brought to a proper incubation temperature. This feature can be used to take a sample at site and forward a proper sample to a lab. Or, if an abnormal field result is observed, the IME.Test™ Ampoule contents can be used for additional analysis (Biological or Chemical).

PRIVATE LABEL/CUSTOM TEST KITS: Private label and/or Customized TEST KITS are available upon request.


Automatic Incubator/Analyzer / Automatic Incubator/Analyzer / Coliform / Dental
E. Coli / Iron Bacteria / Micro Inspector / Potable Water / Sulfur Reducing Bacteria / Sulfur Reducing Bacteria-P
Total Microbe Family / Kool Kount / Kool Kount-P / Kool Kount 3.5/ Kool Kount .05 / Total Sulfur Bacteria / Total Sulfur Bacteria-P

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